U-Blot® eNOS Mouse Monoclonal Antibody (Mix)

SKU:BHA21802628
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-NOS3 antibody (Mouse host, monoclonal, Unconjugated). Validated for Western Blot with reactivity to Human, Mouse, Rat, Rabbit.
Clonality Monoclonal
Host Mouse
Reactivity Human, Mouse, Rat, Rabbit
Conjugate Unconjugated
Applications Western Blot
Options selector
Catalog no. Size
WM1068-50 50 uL
WM1068-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target NOS3
Antigen
  • Nitric oxide synthase
  • endothelial
Alternative names NOS3;Nitric oxide synthase, endothelial;Constitutive NOS;cNOS;EC-NOS;Endothelial NOS;eNOS;NOS type III;NOSIII
Gene ID 4846 (Human) | 18127 (Mouse) | 24600 (Rat)
Host Mouse
Clonality
  • Monoclonal
Reactivity
  • Human
  • Mouse
  • Rat
  • Rabbit
Applications
  • Western Blot
Immunogen Recombinant Protein of eNOS
Conjugate
  • Unconjugated
Molecular weight Observed: 130-140 kDa
Purification Affinity Purified
Cellular localization Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
Formulation PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WM1068
Main SKU BHA21802628
Antibody

Product Overview

U-Blot® eNOS Mouse Monoclonal Antibody (Mix) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against NOS3. It has been validated for Western Blot and reacts with Human, Mouse, Rat, Rabbit.

Validated Applications

Target NOS3
Host Mouse
Clonality Monoclonal
Conjugate Unconjugated
Reactivity Human, Mouse, Rat, Rabbit
Applications Western Blot
Purification Affinity Purified
Specificity The antibody detects endogenous eNOS protein.
Molecular Weight Observed: 130-140 kDa
UniProt / Swiss-Prot P29474 (Human) | P70313 (Mouse) | Q62600 (Rat)

Recommended dilutions: WB 1:500-2000

Immunogen / Antigen

Recombinant Protein of eNOS

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat, Rabbit. Confirm suitability for untested species before use.

Scientific Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot. Recommended dilutions: WB 1:500-2000

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat, Rabbit. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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