| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Nitric oxide synthase, endothelial;Constitutive NOS;cNOS;EC-NOS;Endothelial NOS;eNOS;NOS type III;NOSIII |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human eNOS (Phospho-Ser1179) |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® eNOS (Phospho Ser1179) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against NOS3. Recognizes Phospho. It has been validated for Immunohistochemistry, Immunofluorescence, Western Blot and reacts with Human, Mouse, Rat.
Validated Applications
| Target | NOS3 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Immunofluorescence, Western Blot |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous phospho levels of eNOS (Phospho-Ser1179) at Human:S1179, Mouse:S1178, Rat:S1178 |
| Molecular Weight | Observed: 130-140 kDa |
| UniProt / Swiss-Prot | P29474 (Human) |
Recommended dilutions: IHC 1:50-200;WB 1:500-2000;IF 1:50-200
Immunogen / Antigen
Synthesized peptide derived from human eNOS (Phospho-Ser1179)
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
Safety & Handling
This antibody is validated for Immunohistochemistry, Immunofluorescence, Western Blot. Recommended dilutions: IHC 1:50-200;WB 1:500-2000;IF 1:50-200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
