U-Blot® EPAS-1 Rabbit Polyclonal Antibody

SKU:BHA21810262
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-EPAS-1 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA with reactivity to Human, Mouse, Rat.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG
Applications Immunofluorescence, Western Blot, Immunohistochemistry, ELISA
Options selector
Catalog no. Size
WP15385-50 50 uL
WP15385-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target EPAS-1
Antigen
  • Endothelial PAS domain-containing protein 1
Alternative names EPAS1;BHLHE73;HIF2A;MOP2;PASD2;Endothelial PAS domain-containing protein 1;EPAS-1;Basic-helix-loop-helix-PAS protein MOP2;Class E basic helix-loop-helix protein 73;bHLHe73;HIF-1-alpha-like factor;HLF;Hypoxia-inducible factor 2-alpha;HIF-2-alpha;HIF2-alpha;Member of PAS protein 2;PAS domain-containing protein 2
Gene ID 2034 (Human) | 13819 (Mouse) | 29452 (Rat)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Immunofluorescence
  • Western Blot
  • Immunohistochemistry
  • ELISA
Immunogen Synthesized peptide derived from human EPAS-1 around the non-acetylation site of K385.
Conjugate
  • Unconjugated
Molecular weight Calculated: 96 kDa | Observed: 110-120 kDa
Purification Affinity Purified
Cellular localization Nucleus . Nucleus speckle . Colocalizes with HIF3A in the nucleus and speckles. .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP15385
Main SKU BHA21810262
Antibody

Product Overview

U-Blot® EPAS-1 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against EPAS-1. It has been validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA and reacts with Human, Mouse, Rat.

Validated Applications

Target EPAS-1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Immunofluorescence, Western Blot, Immunohistochemistry, ELISA
Purification Affinity Purified
Specificity EPAS-1 Polyclonal Antibody detects endogenous levels of EPAS-1 protein.
Molecular Weight Calculated: 96 kDa | Observed: 110-120 kDa
UniProt / Swiss-Prot Q99814 (Human) | P97481 (Mouse) | Q9JHS1 (Rat)

Recommended dilutions: IF 1:50-200;WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:20000;Not yet tested in other applications

Immunogen / Antigen

Synthesized peptide derived from human EPAS-1 around the non-acetylation site of K385.

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

endothelial PAS domain protein 1(EPAS1) Homo sapiens This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA. Recommended dilutions: IF 1:50-200;WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:20000;Not yet tested in other applications

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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