| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | ESR1;ESR;NR3A1;Estrogen receptor;ER;ER-alpha;Estradiol receptor;Nuclear receptor subfamily 3 group A member 1 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Estrogen Receptor-alpha around the phosphorylation site of Ser305. AA range:276-325 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® ERα (Phospho Ser305) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Estrogen Receptor-α. Recognizes Phospho. It has been validated for Immunofluorescence, ELISA and reacts with Human, Rat, Mouse.
Validated Applications
| Target | Estrogen Receptor-α |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-ERα (S305) Polyclonal Antibody detects endogenous levels of ERα protein only when phosphorylated at S305. |
| Molecular Weight | Calculated: 66 kDa |
| UniProt / Swiss-Prot | P03372 (Human) | P19785 (Mouse) |
Recommended dilutions: IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human Estrogen Receptor-alpha around the phosphorylation site of Ser305. AA range:276-325
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]
Safety & Handling
This antibody is validated for Immunofluorescence, ELISA. Recommended dilutions: IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
