| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | ERCC1;DNA excision repair protein ERCC-1 |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic Peptide of ERCC1 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® ERCC1 Mouse Monoclonal Antibody (1B10) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against ERCC1. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence and reacts with Human.
Validated Applications
| Target | ERCC1 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1B10 |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | The antibody detects endogenous ERCC1 proteins. |
| Molecular Weight | Observed: 36 kDa |
| UniProt / Swiss-Prot | P07992 (Human) | P07903 (Mouse) |
Recommended dilutions: IHC: 100-300;WB 1:1000;IF 1:50-200
Immunogen / Antigen
Synthetic Peptide of ERCC1
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein ge
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence. Recommended dilutions: IHC: 100-300;WB 1:1000;IF 1:50-200
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone 1B10.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
