| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | MAPK3;ERK1;PRKM3;Mitogen-activated protein kinase 3;MAP kinase 3;MAPK 3;ERT2;Extracellular signal-regulated kinase 1;ERK-1;Insulin-stimulated MAP2 kinase;MAP kinase isoform p44;p44-MAPK;Microtubule-associated protein 2 kinase;p |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p44/42 MAP Kinase around the phosphorylation site of Thr202. AA range:169-218 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® ERK 1/2 (Phospho Thr202) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against ERK 1/2. Recognizes Phospho. It has been validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | ERK 1/2 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunofluorescence, Western Blot, Immunohistochemistry, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-ERK 1/2 (T202) Polyclonal Antibody detects endogenous levels of ERK 1/2 protein only when phosphorylated at T202. |
| Molecular Weight | Observed: 44 kDa,42 kDa |
| UniProt / Swiss-Prot | P27361;P28482 (Human) | P21708;P63086 (Rat) |
Recommended dilutions: IF 1:50-200;WB 1:500-2000;IHC 1:50-300
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human p44/42 MAP Kinase around the phosphorylation site of Thr202. AA range:169-218
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA. Recommended dilutions: IF 1:50-200;WB 1:500-2000;IHC 1:50-300
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
