U-Blot® ERK 1 Rabbit Polyclonal Antibody

SKU:BHA21806808
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-ERK 1 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, Immunofluorescence, ELISA with reactivity to Human, Mouse, Rat.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, Immunofluorescence, ELISA
Options selector
Catalog no. Size
WP12245-50 50 uL
WP12245-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target ERK 1
Antigen
  • Mitogen-activated protein kinase 3
Alternative names MAPK3;ERK1;PRKM3;Mitogen-activated protein kinase 3;MAP kinase 3;MAPK 3;ERT2;Extracellular signal-regulated kinase 1;ERK-1;Insulin-stimulated MAP2 kinase;MAP kinase isoform p44;p44-MAPK;Microtubule-associated protein 2 kinase;p
Gene ID 5595 (Human) | 26417 (Mouse) | 50689 (Rat)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • Immunofluorescence
  • ELISA
Immunogen The antiserum was produced against synthesized peptide derived from human p44 MAPK. AA range:161-210
Conjugate
  • Unconjugated
Molecular weight Observed: 45 kDa
Purification Affinity Purified
Cellular localization Cytoplasm . Nucleus. Membrane, caveola . Cell junction, focal adhesion . Autophosphorylation at Thr-207 promotes nuclear localization (PubMed:19060905). PEA15-binding redirects the biological outcome of MAPK3 kinase-signaling by sequestering MAPK3 into the cytoplasm (By similarity). .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP12245
Main SKU BHA21806808
Antibody

Product Overview

U-Blot® ERK 1 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against ERK 1. It has been validated for Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.

Validated Applications

Target ERK 1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Western Blot, Immunofluorescence, ELISA
Purification Affinity Purified
Specificity ERK 1 Polyclonal Antibody detects endogenous levels of ERK 1 protein.
Molecular Weight Observed: 45 kDa
UniProt / Swiss-Prot P27361 (Human) | Q63844 (Mouse) | P21708 (Rat)

Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:40000;Not yet tested in other applications.

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human p44 MAPK. AA range:161-210

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:40000;Not yet tested in other applications.

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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