| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | EDN1;Endothelin-1;Preproendothelin-1;PPET1 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human EDN1. AA range:131-180 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® ET-1 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against ET-1. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Rat, Mouse.
Validated Applications
| Target | ET-1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | ET-1 Polyclonal Antibody detects endogenous levels of ET-1 protein. |
| Molecular Weight | Observed: 25 kDa |
| UniProt / Swiss-Prot | P05305 (Human) | P22387 (Mouse) |
Recommended dilutions: IHC: 100-300;WB 1:500-1:2000;ELISA 1:20000;IF 1:50-200
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from the Internal region of human EDN1. AA range:131-180
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a preproprotein that is proteolytically processed to generate a secreted peptide that belongs to the endothelin/sarafotoxin family. This peptide is a potent vasoconstrictor and its cognate receptors are therapeutic targets in the treatment of pulmonary arterial hypertension. Aberrant expression of this gene may promote tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC: 100-300;WB 1:500-1:2000;ELISA 1:20000;IF 1:50-200
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
