| Field | Specification |
|---|---|
| Target | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Protein of EYFP |
| Conjugate | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® EYFP-tag Mouse Monoclonal Antibody (Mix) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against EYFP. It has been validated for Western Blot, Immunofluorescence and reacts with the listed species.
Validated Applications
| Target | EYFP |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Species independent |
| Applications | Western Blot, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | The antibody detects EYFP YFP CFP morange tag fusion proteins. It is also reactive against GFP tag weakly |
Recommended dilutions: WB 1:5000;IF 1:300-500
Immunogen / Antigen
Recombinant Protein of EYFP
Scientific Background
Enhanced Yellow florescent protein.Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein, derived from Aequorea victoria. Its excitation peak is 514nm and its emission peak is 527nm. Like green fluorescent protein (GFP), it is a useful tool in cell and molecular biology, usually explored using fluorescence microscopy. Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (product of the extinction coefficient and quantum yield). Typically, yellow FPs serve as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is mCFP (monomeric cyan FP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore.
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence. Recommended dilutions: WB 1:5000;IF 1:300-500
Confirmed reactivity: the listed species. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
