| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNase IV;FEN-1;FEN1;FEN1_HUMAN;Flap endonuclease 1;Flap structure specific endonuclease 1;Flap structure-specific endonuclease 1;hFEN-1;hFEN1;Maturation factor 1;MF1;Rad2. |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human FEN-1 protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® FEN-1 Mouse Monoclonal Antibody (WM0709) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against FEN-1. It has been validated for Western Blot, Immunofluorescence and reacts with Human, Mouse, Rat, Monkey, Hamster.
Validated Applications
| Target | FEN-1 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Monkey, Hamster |
| Applications | Western Blot, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of FEN-1 and does not cross-react with related proteins. |
| Molecular Weight | Observed: 45 kDa |
| UniProt / Swiss-Prot | P39748 (Human) | P39749 (Mouse) |
Recommended dilutions: WB 1:1000;ICC 1:400
Immunogen / Antigen
Purified recombinant human FEN-1 protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Monkey, Hamster. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence. Recommended dilutions: WB 1:1000;ICC 1:400
Confirmed reactivity: Human, Mouse, Rat, Monkey, Hamster. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
