U-Blot® FGF-1 Rabbit Polyclonal Antibody

SKU:BHA21806863
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-FGF-1 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human, Mouse, Rat.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG
Applications Immunohistochemistry, Immunofluorescence, ELISA
Options selector
Catalog no. Size
WP12295-50 50 uL
WP12295-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target FGF-1
Antigen
  • Fibroblast growth factor 1
Alternative names FGF1;FGFA;Fibroblast growth factor 1;FGF-1;Acidic fibroblast growth factor;aFGF;Endothelial cell growth factor;ECGF;Heparin-binding growth factor 1;HBGF-1
Gene ID 2246 (Human) | 14164 (Mouse) | 25317 (Rat)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Immunohistochemistry
  • Immunofluorescence
  • ELISA
Immunogen The antiserum was produced against synthesized peptide derived from human FGF-1. AA range:7-56
Conjugate
  • Unconjugated
Molecular weight Calculated: 17 kDa
Purification Affinity Purified
Cellular localization Secreted. Cytoplasm. Cytoplasm, cell cortex. Cytoplasm, cytosol. Nucleus. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1 (By similarity). Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59. .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP12295
Main SKU BHA21806863
Antibody

Product Overview

U-Blot® FGF-1 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against FGF-1. It has been validated for Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.

Validated Applications

Target FGF-1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Immunohistochemistry, Immunofluorescence, ELISA
Purification Affinity Purified
Specificity FGF-1 Polyclonal Antibody detects endogenous levels of FGF-1 protein.
Molecular Weight Calculated: 17 kDa
UniProt / Swiss-Prot P05230 (Human) | P61148 (Mouse) | P61149 (Rat)

Recommended dilutions: IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human FGF-1. AA range:7-56

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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