| Field | Specification |
|---|---|
| Alternative Names | FGF2;FGFB;Fibroblast growth factor 2;FGF-2;Basic fibroblast growth factor;bFGF;Heparin-binding growth factor 2;HBGF-2 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human FGF2. AA range:151-200 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® FGF-2 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against FGF-2. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | FGF-2 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | FGF-2 Polyclonal Antibody detects endogenous levels of FGF-2 protein. |
| Molecular Weight | Observed: 30 kDa |
| UniProt / Swiss-Prot | P09038 (Human) | P15655 (Mouse) | P13109 (Rat) |
Recommended dilutions: WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:10000;IF 1:50-200
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from the Internal region of human FGF2. AA range:151-200
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:10000;IF 1:50-200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
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