| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Fibronectin;FN;Cold-insoluble globulin;CIG;[Cleaved into: Anastellin;Ugl-Y1;Ugl-Y2;Ugl-Y3] |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthetic peptide from human protein at AA range: 2304-2375 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Fibronectin Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Fibronectin. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Rat, Mouse.
Validated Applications
| Target | Fibronectin |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | The antibody detects endogenous Fibronectin |
| Molecular Weight | Calculated: 272 kDa |
| UniProt / Swiss-Prot | P02751 (Human) | P11276 (Mouse) |
Recommended dilutions: IHC 1:50-200;ELISA 1:10000-20000;WB 1:500-2000;IF 1:50-200
Immunogen / Antigen
Synthetic peptide from human protein at AA range: 2304-2375
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:50-200;ELISA 1:10000-20000;WB 1:500-2000;IF 1:50-200
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
