| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | KDR;FLK1;VEGFR2;Vascular endothelial growth factor receptor 2;VEGFR-2;Fetal liver kinase 1;FLK-1;Kinase insert domain receptor;KDR;Protein-tyrosine kinase receptor flk-1;CD antigen CD309 |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant extracellular fragment of human Flk-1 (aa20-764) fused with hIgGFc tag expressed in HEK293 cells. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Flk-1/VEGFR2 Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Flk-1/VEGFR2. It has been validated for Western Blot, ChIP, Immunofluorescence, Flow Cytometry, ELISA and reacts with Human, Fish.
Validated Applications
| Target | Flk-1/VEGFR2 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Fish |
| Applications | Western Blot, ChIP, Immunofluorescence, Flow Cytometry, ELISA |
| Purification | Affinity Purified |
| Specificity | Flk-1 Monoclonal Antibody detects endogenous levels of Flk-1 protein. |
| Molecular Weight | Calculated: 152 kDa |
| UniProt / Swiss-Prot | P35968 (Human) | P35918 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant extracellular fragment of human Flk-1 (aa20-764) fused with hIgGFc tag expressed in HEK293 cells.
Reactivity Notes
Confirmed cross-reactivity: Human, Fish. Confirm suitability for untested species before use.
Scientific Background
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]
Safety & Handling
This antibody is validated for Western Blot, ChIP, Immunofluorescence, Flow Cytometry, ELISA. Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;Not yet tested in other applications.
Confirmed reactivity: Human, Fish. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
