| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | FLT3;CD135;FLK2;STK1;Receptor-type tyrosine-protein kinase FLT3;FL cytokine receptor;Fetal liver kinase-2;FLK-2;Fms-like tyrosine kinase 3;FLT-3;Stem cell tyrosine kinase 1;STK-1;CD antigen CD135 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FLT3 around the phosphorylation site of Tyr599. AA range:565-614 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® FLT3 (Phospho Tyr599) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against FLT3. Recognizes Phospho. It has been validated for Western Blot, ELISA and reacts with Human, Mouse, Monkey.
Validated Applications
| Target | FLT3 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Monkey |
| Applications | Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-Flt3 (Y599) Polyclonal Antibody detects endogenous levels of Flt3 protein only when phosphorylated at Y599. |
| Molecular Weight | Observed: 160 kDa |
| UniProt / Swiss-Prot | P36888 (Human) | Q00342 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;ELISA 1:5000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human FLT3 around the phosphorylation site of Tyr599. AA range:565-614
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Monkey. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. [provided by RefSeq, Jan 2015]
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-1:2000;ELISA 1:5000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Monkey. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
