U-Blot® FOXO1(C-term) Mouse Monoclonal Antibody

SKU:BHA21802185
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Suppliers
UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-FoxO1 antibody (Mouse host, monoclonal, Unconjugated). Validated for Western Blot with reactivity to Human.
Clonality Monoclonal
Host Mouse
Reactivity Human
Conjugate Unconjugated
Applications Western Blot
Options selector
Catalog no. Size
WM0607-50 50 uL
WM0607-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target FoxO1
Alternative names FKH 1;FKH1;FKH1;FKHR;FKHR;Forkhead;Drosophila;homolog 1;rhabdomyosarcoma;Forkhead;Drosophila;homolog 1;rhabdomyosarcoma;Forkhead box O1;Forkhead box protein O1;Forkhead box protein O1A;Forkhead in rhabdomyosarcoma;Forkhead, Drosophila, homolog of, in rhabdomyosarcoma;FOXO1;FOXO1;FOXO1_HUMAN;FOXO1A;OTTHUMP00000018301.
Gene ID 2308 (Human)
Host Mouse
Clonality
  • Monoclonal
Reactivity
  • Human
Applications
  • Western Blot
Immunogen Purified recombinant human FOXO1(C-terminus) protein fragments expressed in E.coli.
Conjugate
  • Unconjugated
Molecular weight Observed: 70 kDa
Purification Affinity Purified
Cellular localization Cytoplasm . Nucleus . Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells (PubMed:11311120, PubMed:12228231, PubMed:19221179, PubMed:21245099, PubMed:20543840, PubMed:25009184). In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Serum deprivation increases localization to the nucleus, leading to activate expression of SOX9 and subsequent chondrogenesis (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway (PubMed:11237865, PubMed:12228231). Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation (PubMed:18356527). Phosphorylation by NLK results in nuclear export (By similarity). Translocates to the nucleus upon oxidative stress-induced phosphorylation at Ser-212 by STK4/MST1 (PubMed:19221179, PubMed:21245099). SGK1-mediated phosphorylation also results in nuclear translocation (By similarity). Retained in the nucleus under stress stimuli including oxidative stress, nutrient deprivation or nitric oxide (By similarity). Retained in the nucleus on methylation (By similarity). PPIA/CYPA stimulates its nuclear accumulation (PubMed:31063815). Deacetylation by SIRT6, promotes its translocation into the cytoplasm (PubMed:25009184). .
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WM0607
Main SKU BHA21802185
Antibody

Product Overview

U-Blot® FOXO1(C-term) Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against FoxO1. It has been validated for Western Blot and reacts with Human.

Validated Applications

Target FoxO1
Host Mouse
Clonality Monoclonal
Conjugate Unconjugated
Reactivity Human
Applications Western Blot
Purification Affinity Purified
Specificity This antibody detects endogenous levels of FOXO1(C-terminus) and does not cross-react with related proteins.
Molecular Weight Observed: 70 kDa
UniProt / Swiss-Prot Q12778 (Human) | Q9R1E0 (Mouse)

Recommended dilutions: WB 1:1000

Immunogen / Antigen

Purified recombinant human FOXO1(C-terminus) protein fragments expressed in E.coli.

Reactivity Notes

Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.

Scientific Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot. Recommended dilutions: WB 1:1000

What species does it react with?

Confirmed reactivity: Human. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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