| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | GAPDH |
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| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Purification | |
| Cellular localization | |
| Concentration | |
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| Catalog no. (Mfr.) | |
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Product Overview
U-Blot® GAPDH Mouse Monoclonal Antibody (2B8), Cy5 Conjugated is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against GAPDH. It has been validated for Western Blot, Immunofluorescence, Immunohistochemistry and reacts with Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep, Insect, Yeast.
Validated Applications
| Target | GAPDH |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2B8 |
| Isotype | IgG1 |
| Conjugate | Cy5 |
| Reactivity | Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep, Insect, Yeast |
| Applications | Western Blot, Immunofluorescence, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | GAPDH Monoclonal Antibody(2B8) Cy5 Conjugated specially designed for your Immunofluorescence analysis. |
| UniProt / Swiss-Prot | P04406 (Human) |
Recommended dilutions: Optimal working dilutions should be determined experimentally by the investigator;Suggested starting dilutions are as follows:IHC 1:200;IF 1:200.
Immunogen / Antigen
Glyceraldehyde-3-phosphate dehydrogenase
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep, Insect, Yeast. Confirm suitability for untested species before use.
Scientific Background
glyceraldehyde-3-phosphate dehydrogenase(GAPDH) Homo sapiens This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferri
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, Immunohistochemistry. Recommended dilutions: Optimal working dilutions should be determined experimentally by the investigator;Suggested starting dilutions are as follows:IHC 1:200;IF 1:200.
Confirmed reactivity: Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep, Insect, Yeast. Other species should be validated by the user.
Conjugate: Cy5.
Store as follows: Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Store in dark.
Clone 2B8.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
