| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | GAPDH;GAPD;CDABP0047;OK/SW-cl.12;Glyceraldehyde-3-phosphate dehydrogenase;GAPDH;Peptidyl-cysteine S-nitrosylase GAPDH |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic Peptide of human GAPDH AA range: 200-300 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® GAPDH (PTR2304) Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against GAPDH. It has been validated for Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat, Dog, Monkey, Rabbit, Pig, Bovine.
Validated Applications
| Target | GAPDH |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PTR2304 |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Dog, Monkey, Rabbit, Pig, Bovine |
| Applications | Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | This antibody detects endogenous levels of GAPDH protein. |
| Molecular Weight | Calculated: 38 kDa | Observed: 38 kDa |
| UniProt / Swiss-Prot | P04406 (Human) | P16858 (Mouse) | P04797 (Rat) |
Recommended dilutions: WB 1:10000-50000;IF 1:100-500;ELISA 1:50000-500000
Immunogen / Antigen
Synthetic Peptide of human GAPDH AA range: 200-300
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Dog, Monkey, Rabbit, Pig, Bovine. Confirm suitability for untested species before use.
Scientific Background
glyceraldehyde-3-phosphate dehydrogenase(GAPDH) Homo sapiens This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferri
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA. Recommended dilutions: WB 1:10000-50000;IF 1:100-500;ELISA 1:50000-500000
Confirmed reactivity: Human, Mouse, Rat, Dog, Monkey, Rabbit, Pig, Bovine. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PTR2304.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
