U-Blot® GAPDH Rabbit Monoclonal Antibody (PT0004R)

SKU:BHA21800001
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-GAPDH antibody (Rabbit host, monoclonal, Unconjugated). Validated for Western Blot, ELISA with reactivity to Human, Mouse, Rat, Rabbit.
Clonality Monoclonal
Host Rabbit
Reactivity Human, Mouse, Rat, Rabbit, Dog, Monkey
Conjugate Unconjugated
Isotype IgG, Kappa
Clone PT0004R
Applications Western Blot, ELISA
Options selector
Catalog no. Size
WR4001-50 50 uL
WR4001-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target GAPDH
Antigen
  • Glyceraldehyde-3-phosphate dehydrogenase
Alternative names GAPDH;GAPD;CDABP0047;OK/SW-cl.12;Glyceraldehyde-3-phosphate dehydrogenase;GAPDH;Peptidyl-cysteine S-nitrosylase GAPDH
Gene ID 2597 (Human)
Host Rabbit
Clone PT0004R
Clonality
  • Monoclonal
Isotype
  • IgG
  • Kappa
Reactivity
  • Human
  • Mouse
  • Rat
  • Rabbit
  • Dog
  • Monkey
Applications
  • Western Blot
  • ELISA
Conjugate
  • Unconjugated
Molecular weight Calculated: 37 kDa | Observed: 37 kDa
Purification Protein A
Cellular localization Cytoplasm, cytosol . Nucleus . Cytoplasm, perinuclear region . Membrane . Cytoplasm, cytoskeleton . Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions (PubMed:12829261). .
Formulation PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WR4001
Main SKU BHA21800001
Antibody

Product Overview

U-Blot® GAPDH Rabbit Monoclonal Antibody (PT0004R) is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against GAPDH. It has been validated for Western Blot, ELISA and reacts with Human, Mouse, Rat, Rabbit, Dog, Monkey.

Validated Applications

Target GAPDH
Host Rabbit
Clonality Monoclonal
Clone PT0004R
Isotype IgG, Kappa
Conjugate Unconjugated
Reactivity Human, Mouse, Rat, Rabbit, Dog, Monkey
Applications Western Blot, ELISA
Purification Protein A
Specificity Endogenous
Molecular Weight Calculated: 37 kDa | Observed: 37 kDa
UniProt / Swiss-Prot P04406 (Human) | P16858 (Mouse)

Recommended dilutions: WB 1:500-2000;ELISA 1:20000

Immunogen / Antigen

Glyceraldehyde-3-phosphate dehydrogenase

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat, Rabbit, Dog, Monkey. Confirm suitability for untested species before use.

Scientific Background

glyceraldehyde-3-phosphate dehydrogenase(GAPDH) Homo sapiens This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferri

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-2000;ELISA 1:20000

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat, Rabbit, Dog, Monkey. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

What is the clone number?

Clone PT0004R.

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