U-Blot® GAPDH Rabbit Polyclonal Antibody (WP5859)

SKU:BHA21817137
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-GAPDH antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot with reactivity to Zebrafish.
Clonality Polyclonal
Host Rabbit
Reactivity Zebrafish
Conjugate Unconjugated
Isotype IgG
Applications Western Blot
Options selector
Catalog no. Size
WP5859-50 50 uL
WP5859-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target GAPDH
Antigen
  • Glyceraldehyde-3-phosphate dehydrogenase
Alternative names GAPDH;GAPD;CDABP0047;OK/SW-cl.12;Glyceraldehyde-3-phosphate dehydrogenase;GAPDH;Peptidyl-cysteine S-nitrosylase GAPDH
Gene ID 2597 (Human) | 100042025 (Mouse) | 24383 (Rat)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Zebrafish
Applications
  • Western Blot
Immunogen Recombinant Protein of Glyceraldehyde-3-phosphate dehydrogenase
Conjugate
  • Unconjugated
Molecular weight Observed: 37 kDa
Purification Affinity Purified
Cellular localization Cytoplasm, cytosol . Nucleus . Cytoplasm, perinuclear region . Membrane . Cytoplasm, cytoskeleton . Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions (PubMed:12829261). .
Formulation PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP5859
Main SKU BHA21817137
Antibody

Product Overview

U-Blot® GAPDH Rabbit Polyclonal Antibody (WP5859) is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against GAPDH. It has been validated for Western Blot and reacts with Zebrafish.

Validated Applications

Target GAPDH
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Zebrafish
Applications Western Blot
Purification Affinity Purified
Specificity The antibody detects Zebrafish endogenous GAPDH protein.
Molecular Weight Observed: 37 kDa
UniProt / Swiss-Prot P04406 (Human) | P16858 (Mouse) | P04797 (Rat)

Recommended dilutions: WB 1:2000-5000

Immunogen / Antigen

Recombinant Protein of Glyceraldehyde-3-phosphate dehydrogenase

Reactivity Notes

Confirmed cross-reactivity: Zebrafish. Confirm suitability for untested species before use.

Scientific Background

glyceraldehyde-3-phosphate dehydrogenase(GAPDH) Homo sapiens This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferri

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot. Recommended dilutions: WB 1:2000-5000

What species does it react with?

Confirmed reactivity: Zebrafish. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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