| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Protein of GFP |
| Conjugate | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® GFP-Tag Mouse Monoclonal Antibody (Mix) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against GFP-Tag. It has been validated for Western Blot, Immunofluorescence, Immunoprecipitation and reacts with the listed species.
Validated Applications
| Target | GFP-Tag |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Species independent |
| Applications | Western Blot, Immunofluorescence, Immunoprecipitation |
| Purification | Affinity Purified |
| Specificity | GFP antibody is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP and EGFP. |
Recommended dilutions: WB 1:5000;IP 1:200;IF 1:50-200
Immunogen / Antigen
Recombinant Protein of GFP
Scientific Background
Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, Immunoprecipitation. Recommended dilutions: WB 1:5000;IP 1:200;IF 1:50-200
Confirmed reactivity: the listed species. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
