U-Blot® GRP78/BiP Mouse Monoclonal Antibody (8G3)

SKU:BHA21802707
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-HSP A5/GRP78 antibody (Mouse host, monoclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence with reactivity to Human, Rat.
Clonality Monoclonal
Host Mouse
Reactivity Human, Rat
Conjugate Unconjugated
Clone 8G3
Applications Western Blot, Immunohistochemistry, Immunofluorescence
Options selector
Catalog no. Size
WM1152-50 50 uL
WM1152-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target HSP A5/GRP78
Antigen
  • 78 kDa glucose-regulated protein (GRP-78) (Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78) (Heat shock 70 kDa protein 5) (Immunoglobulin heavy chain-binding protein) (BiP)
Alternative names HSPA5;GRP78;78 kDa glucose-regulated protein;GRP-78;Endoplasmic reticulum lumenal Ca;2+;-binding protein grp78;Heat shock 70 kDa protein 5;Immunoglobulin heavy chain-binding protein;BiP
Gene ID 3309 (Human)
Host Mouse
Clone 8G3
Clonality
  • Monoclonal
Reactivity
  • Human
  • Rat
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
Immunogen Synthetic Peptide of GRP78/Bip
Conjugate
  • Unconjugated
Molecular weight Observed: 78 kDa
Purification Affinity Purified
Cellular localization Endoplasmic reticulum lumen . Melanosome . Cytoplasm . Cell surface . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545). Localizes to the cell surface of epithelial cells in response to high levels of free iron (PubMed:20484814, PubMed:24355926, PubMed:27159390). .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WM1152
Main SKU BHA21802707
Antibody

Product Overview

U-Blot® GRP78/BiP Mouse Monoclonal Antibody (8G3) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against HSP A5/GRP78. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence and reacts with Human, Rat.

Validated Applications

Target HSP A5/GRP78
Host Mouse
Clonality Monoclonal
Clone 8G3
Conjugate Unconjugated
Reactivity Human, Rat
Applications Western Blot, Immunohistochemistry, Immunofluorescence
Purification Affinity Purified
Specificity GRP78/Bip protein detects endogenous levels of GRP78/Bip
Molecular Weight Observed: 78 kDa
UniProt / Swiss-Prot P11021 (Human) | P20029 (Mouse) | P06761 (Rat)

Recommended dilutions: WB 1:1000-2000;IHC 1:100-200;IF 1:50-200

Immunogen / Antigen

Synthetic Peptide of GRP78/Bip

Reactivity Notes

Confirmed cross-reactivity: Human, Rat. Confirm suitability for untested species before use.

Scientific Background

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence. Recommended dilutions: WB 1:1000-2000;IHC 1:100-200;IF 1:50-200

What species does it react with?

Confirmed reactivity: Human, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

What is the clone number?

Clone 8G3.

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