| Field | Specification |
|---|---|
| Alternative Names | 78 kDa glucose-regulated protein;GRP-78;Endoplasmic reticulum lumenal Ca;2+;-binding protein grp78;Heat shock 70 kDa protein 5;Immunoglobulin heavy chain-binding protein;BiP |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthetic peptide from human protein at AA range: 505-570 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® GRP78 BiP Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against HSP A5/GRP78. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat, Fish.
Validated Applications
| Target | HSP A5/GRP78 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Fish |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | The antibody detects endogenous GRP78 BiP |
| Molecular Weight | Observed: 78 kDa |
| UniProt / Swiss-Prot | P11021 (Human) | P20029 (Mouse) | P06761 (Rat) |
Recommended dilutions: WB 1:500-1:2000;IHC-f:1:50-300;ELISA 1:10000-20000;IF 1:50-200
Immunogen / Antigen
Synthetic peptide from human protein at AA range: 505-570
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Fish. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC-f:1:50-300;ELISA 1:10000-20000;IF 1:50-200
Confirmed reactivity: Human, Mouse, Rat, Fish. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
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