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Product Overview
U-Blot® HA-Tag Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against HA-Tag. It has been validated for Western Blot, Immunofluorescence, ELISA and reacts with the listed species.
Validated Applications
| Target | HA-Tag |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0523R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Applications | Western Blot, Immunofluorescence, ELISA |
| Purification | Protein A |
| Specificity | The antibody can detect HA-Tag fusion proteins. |
| Molecular Weight | Calculated: 50 kDa | Observed: 55 kDa |
Recommended dilutions: WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Immunogen / Antigen
HA-Tag
Scientific Background
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA. Recommended dilutions: WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Confirmed reactivity: the listed species. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0523R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.