| Field | Specification |
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| Target | |
| Antigen | |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human hCG AA range: 25-100 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® hCG α (ABT-HCG) Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against hCG. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | hCG |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT-HCG |
| Isotype | IgG2a, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize α subunits of human CG, LH, FSH and TSH proteins. In western blotting of Hela cell lysate, the antibody can label a 18 kDa band. |
| Molecular Weight | Calculated: 13 kDa | Observed: 13 kDa |
| UniProt / Swiss-Prot | P01215 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human hCG AA range: 25-100
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT-HCG.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.