U-Blot® HECAM Rabbit Polyclonal Antibody

SKU:BHA21814052
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-HECAM antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, ELISA with reactivity to Human, Mouse.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, ELISA
Options selector
Catalog no. Size
WP2774-50 50 uL
WP2774-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target HECAM
Antigen
  • Hepatocyte cell adhesion molecule (Protein hepaCAM)
Gene ID 220296 (Human)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
Applications
  • Western Blot
  • ELISA
Immunogen Synthesized peptide derived from human protein . at AA range: 10-90
Conjugate
  • Unconjugated
Molecular weight Observed: 45 kDa
Purification Affinity Purified
Cellular localization Cytoplasm . Membrane; Single-pass type I membrane protein; Cytoplasmic side . In MCF-7 breast carcinoma and hepatic Hep 3B2.1-7 and Hep-G2 cell lines, localization of HEPACAM is cell density-dependent. In well spread cells, localized to punctate structures in the perinuclear membrane, cytoplasm, and at cell surface of protusions. In confluent cells, localized predominantly to the cytoplasmic membrane, particularly in areas of cell-cell contacts. Colocalizes with CDH1.
Concentration 1 mg/ml
Formulation PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP2774
Main SKU BHA21814052
Antibody

Product Overview

U-Blot® HECAM Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against HECAM. It has been validated for Western Blot, ELISA and reacts with Human, Mouse.

Validated Applications

Target HECAM
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse
Applications Western Blot, ELISA
Purification Affinity Purified
Specificity HECAM Polyclonal Antibody detects endogenous levels of protein.
Molecular Weight Observed: 45 kDa
UniProt / Swiss-Prot Q14CZ8 (Human) | Q640R3 (Mouse)

Recommended dilutions: WB 1:500-2000;ELISA 1:5000-20000

Immunogen / Antigen

Synthesized peptide derived from human protein . at AA range: 10-90

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.

Scientific Background

The protein encoded by this gene is a single-pass type I membrane protein that localizes to the cytoplasmic side of the cell membrane. The encoded protein acts as a homodimer and is involved in cell motility and cell-matrix interactions. The expression of this gene is downregulated or undetectable in many cancer cell lines, so this may be a tumor suppressor gene. [provided by RefSeq, Jul 2011]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-2000;ELISA 1:5000-20000

What species does it react with?

Confirmed reactivity: Human, Mouse. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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