| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Receptor tyrosine-protein kinase erbB-2;Metastatic lymph node gene 19 protein;MLN 19;Proto-oncogene Neu;Proto-oncogene c-ErbB-2;Tyrosine kinase-type cell surface receptor HER2;p185erbB2;CD antigen CD340 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human HER2 (Phospho-Tyr1139) |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® HER2 (Phospho Tyr1139) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against HER2. Recognizes Phospho. It has been validated for Immunohistochemistry, Immunofluorescence, Western Blot and reacts with Human, Mouse, Rat.
Validated Applications
| Target | HER2 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Immunofluorescence, Western Blot |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous phospho levels of HER2 (Phospho-Tyr1139) at Human:Y1139, Mouse:Y1140, Rat:Y1141 |
| Molecular Weight | Observed: 180 kDa |
| UniProt / Swiss-Prot | P04626 (Human) |
Recommended dilutions: IHC 1:50-200;WB 1:500-2000;IF 1:50-200
Immunogen / Antigen
Synthesized peptide derived from human HER2 (Phospho-Tyr1139)
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding d
Safety & Handling
This antibody is validated for Immunohistochemistry, Immunofluorescence, Western Blot. Recommended dilutions: IHC 1:50-200;WB 1:500-2000;IF 1:50-200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
