| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | H1K25ME1;HIST1H1A;H1F1;Histone H1.1;Histone H1a;HIST1H1C;H1F2;Histone H1.2;Histone H1c;Histone H1d;Histone H1s-1;HIST1H1D;H1F3;Histone H1.3;Histone H1c;Histone H1s-2;HIST1H1E;H1F4;Histone H1.4;Histone H1b;Histone H1s-4 |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic Peptide of Histone H1 (Mono Methyl Lys25) |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Histone H1 (Mono Methyl Lys25) Mouse Polyclonal Antibody is a mouse polyclonal antibody from UcallM Biosciences Inc, raised against Histone H1. Recognizes Mono Methyl. It has been validated for Western Blot and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Histone H1 |
|---|---|
| Host | Mouse |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot |
| Purification | Affinity Purified |
| Specificity | The antibody detects endogenous Histone H1 (Mono Methyl Lys25) protein. |
| Molecular Weight | Observed: 17-25 kDa |
| UniProt / Swiss-Prot | Q02539;P16403;P16402;P10412 (Human) | D4A3K5;P15865 (Rat) |
Recommended dilutions: WB 1:500-1000
Immunogen / Antigen
Synthetic Peptide of Histone H1 (Mono Methyl Lys25)
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]
Safety & Handling
This antibody is validated for Western Blot. Recommended dilutions: WB 1:500-1000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
