| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | HIST1H1B;H1F5;Histone H1.5;Histone H1a;Histone H1b;Histone H1s-3;HIST1H1D;H1F3;Histone H1.3;Histone H1c;Histone H1s-2;HIST1H1E;H1F4;Histone H1.4;Histone H1b;Histone H1s-4 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H1 around the phosphorylation site of Thr17. AA range:1-50 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Histone H1 (Phospho Thr17) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Histone H1. Recognizes Phospho. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Monkey.
Validated Applications
| Target | Histone H1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Monkey |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-Histone H1 (T17) Polyclonal Antibody detects endogenous levels of Histone H1 protein only when phosphorylated at T17. |
| Molecular Weight | Observed: 31 kDa |
| UniProt / Swiss-Prot | P16401;P16402;P10412 (Human) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human Histone H1 around the phosphorylation site of Thr17. AA range:1-50
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Monkey. Confirm suitability for untested species before use.
Scientific Background
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Monkey. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
