| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | H2BFS;Histone H2B type F-S;Histone H2B.s;H2B/s;H2BK21AC |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic Acetyl peptide from human protein at AA range: 21 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Histone H2B (Acetyl Lys21) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Histone H2B. Recognizes Acetyl. It has been validated for Western Blot, ELISA and reacts with Human, Rat, Mouse.
Validated Applications
| Target | Histone H2B |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | The antibody detects endogenous Histone H2B when Acetyl occurs at Lys21 |
| Molecular Weight | Observed: 15 kDa |
| UniProt / Swiss-Prot | Q96A08;P33778;P62807 (Human) |
Recommended dilutions: WB 1:500-2000;ELISA 1:10000-20000
Immunogen / Antigen
Synthetic Acetyl peptide from human protein at AA range: 21
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015]
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-2000;ELISA 1:10000-20000
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
