| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Histone H2B type 1-C/E/F/G/I;Histone H2B.1 A;Histone H2B.a;H2B/a;Histone H2B.g;H2B/g;Histone H2B.h;H2B/h;Histone H2B.k;H2B/k;Histone H2B.l;H2B/l |
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| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
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Product Overview
U-Blot® Histone H2B Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against Histone H2B. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA, ChIP and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Histone H2B |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0420R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA, ChIP |
| Purification | Protein A |
| Specificity | Endogenous |
| Molecular Weight | Calculated: 14 kDa | Observed: 14 kDa |
| UniProt / Swiss-Prot | P62807;P58876;Q93079;O60814;Q99880;Q99879;Q99877;Q5QNW6;P57053 (Human) | Q6ZWY9 (Mouse) |
Recommended dilutions: IHC 1:20000-1:50000;WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;CHIP 1:50-1:100;Cut&Tag 1:50-1:100
Immunogen / Antigen
Histone H2B (Acetyl Lys35)
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Two transcripts that encode the same protein have been identified for this gene, which is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA, ChIP. Recommended dilutions: IHC 1:20000-1:50000;WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;CHIP 1:50-1:100;Cut&Tag 1:50-1:100
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0420R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
