| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | HMGB1;HMG1;High mobility group protein B1;High mobility group protein 1;HMG-1 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from the N-terminal region of human HMG-1. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® HMG-1 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against HMGB1. It has been validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | HMGB1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunofluorescence, Western Blot, Immunohistochemistry, ELISA |
| Purification | Affinity Purified |
| Specificity | HMG-1 Polyclonal Antibody detects endogenous levels of HMG-1 protein. |
| Molecular Weight | Observed: 30 kDa |
| UniProt / Swiss-Prot | P09429 (Human) | P63158 (Mouse) | P63159 (Rat) |
Recommended dilutions: IF 1:50-200;WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:20000;Not yet tested in other applications
Immunogen / Antigen
Synthesized peptide derived from the N-terminal region of human HMG-1.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
Safety & Handling
This antibody is validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA. Recommended dilutions: IF 1:50-200;WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:20000;Not yet tested in other applications
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
