U-Blot® HSF1 (Phospho Thr142) Rabbit Polyclonal Antibody

SKU:BHA21804400
New
—
Suppliers
UcallM Biosciences Inc
UcallM Biosciences Inc
Details Products
Overview
Click light‑blue chips for details
Anti-HSF1 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human, Mouse.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse
Conjugate Unconjugated
Isotype IgG
Applications Immunohistochemistry, Immunofluorescence, ELISA
Options selector
Catalog no. Size
WP10055-50 50 uL
WP10055-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target HSF1
Antigen
  • Heat shock factor protein 1
Alternative names HSF1;HSTF1;Heat shock factor protein 1;HSF 1;Heat shock transcription factor 1;HSTF 1
Gene ID 3297 (Human) | 15499 (Mouse)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
Applications
  • Immunohistochemistry
  • Immunofluorescence
  • ELISA
Immunogen The antiserum was produced against synthesized peptide derived from human HSF1 around the phosphorylation site of Thr142. AA range:108-157
Conjugate
  • Unconjugated
Molecular weight Calculated: 31 kDa
Purification Affinity Purified
Cellular localization Nucleus . Cytoplasm . Nucleus, nucleoplasm . Cytoplasm, perinuclear region . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Chromosome, centromere, kinetochore . The monomeric form is cytoplasmic in unstressed cells (PubMed:8455624, PubMed:26159920). Predominantly nuclear protein in both unstressed and heat shocked cells (PubMed:10413683, PubMed:10359787). Translocates in the nucleus upon heat shock (PubMed:8455624). Nucleocytoplasmic shuttling protein (PubMed:26159920). Colocalizes with IER5 in the nucleus (PubMed:27354066). Colocalizes with BAG3 to the nucleus upon heat stress (PubMed:8455624, PubMed:26159920). Localizes in subnuclear granules called nuclear stress bodies (nSBs) upon heat shock (PubMed:11447121, PubMed:11514557, PubMed:10359787, PubMed:25963659, PubMed:10747973, PubMed:24581496, PubMed:19229036). Colocalizes with SYMPK and SUMO1 in nSBs upon heat shock (PubMed:11447121, PubMed:12665592, PubMed:11514557, PubMed:14707147, PubMed:10359787). Colocalizes with PRKACA/PKA in the nucleus and nSBs upon heat shock (PubMed:21085490). Relocalizes from the nucleus to the cytoplasm during the attenuation and recovery phase period of the heat shock response (PubMed:26159920). Translocates in the cytoplasm in a YWHAE- and XPO1/CRM1-dependent manner (PubMed:12917326). Together with histone H2AX, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) (PubMed:26359349). Colocalizes with calcium-responsive transactivator SS18L1 at kinetochore region on the mitotic chromosomes (PubMed:18794143). Colocalizes with gamma tubulin at centrosome (PubMed:18794143). Localizes at spindle pole in metaphase (PubMed:18794143). Colocalizes with PLK1 at spindle poles during prometaphase (PubMed:18794143). .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP10055
Main SKU BHA21804400
Antibody

Product Overview

U-Blot® HSF1 (Phospho Thr142) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against HSF1. Recognizes Phospho. It has been validated for Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse.

Validated Applications

Target HSF1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse
Applications Immunohistochemistry, Immunofluorescence, ELISA
Purification Affinity Purified
Specificity Phospho-HSF1 (T142) Polyclonal Antibody detects endogenous levels of HSF1 protein only when phosphorylated at T142.
Molecular Weight Calculated: 31 kDa
UniProt / Swiss-Prot Q00613 (Human) | P38532 (Mouse)

Recommended dilutions: IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human HSF1 around the phosphorylation site of Thr142. AA range:108-157

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.

Scientific Background

heat shock transcription factor 1(HSF1) Homo sapiens The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phsphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Aug 2016]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200

What species does it react with?

Confirmed reactivity: Human, Mouse. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

Get a Quote

Please use this form for bulk quantity requests or customized products.

Contact Information

Product Information

Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today

Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today