U-Blot® HSP27 Mouse Monoclonal Antibody (Mix-mA)

SKU:BHA21802697
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-HSP27 antibody (Mouse host, monoclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence with reactivity to Human.
Clonality Monoclonal
Host Mouse
Reactivity Human
Conjugate Unconjugated
Applications Western Blot, Immunohistochemistry, Immunofluorescence
Options selector
Catalog no. Size
WM1141-50 50 uL
WM1141-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target HSP27
Antigen
  • Heat shock protein beta-1 (HspB1) (28 kDa heat shock protein) (Estrogen-regulated 24 kDa protein) (Heat shock 27 kDa protein) (HSP 27) (Stress-responsive protein 27) (SRP27)
Alternative names Heat shock protein beta-1;HspB1;28 kDa heat shock protein;Estrogen-regulated 24 kDa protein;Heat shock 27 kDa protein;HSP 27;Stress-responsive protein 27;SRP27
Gene ID 3315 (Human)
Host Mouse
Clonality
  • Monoclonal
Reactivity
  • Human
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
Immunogen Recombinant Protein of HSP27
Conjugate
  • Unconjugated
Molecular weight Observed: 27 kDa
Purification Affinity Purified
Cellular localization Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, spindle . Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WM1141
Main SKU BHA21802697
Antibody

Product Overview

U-Blot® HSP27 Mouse Monoclonal Antibody (Mix-mA) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against HSP27. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence and reacts with Human.

Validated Applications

Target HSP27
Host Mouse
Clonality Monoclonal
Conjugate Unconjugated
Reactivity Human
Applications Western Blot, Immunohistochemistry, Immunofluorescence
Purification Affinity Purified
Specificity Antibody can detects endogenous HSP27 protein.
Molecular Weight Observed: 27 kDa
UniProt / Swiss-Prot P04792 (Human) | P14602 (Mouse)

Recommended dilutions: WB 1:1000-1:3000;IHC 1:50-1:200;IF 1:50-200

Immunogen / Antigen

Recombinant Protein of HSP27

Reactivity Notes

Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.

Scientific Background

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence. Recommended dilutions: WB 1:1000-1:3000;IHC 1:50-1:200;IF 1:50-200

What species does it react with?

Confirmed reactivity: Human. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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