| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | HSPB1;HSP27;HSP28;Heat shock protein beta-1;HspB1;28 kDa heat shock protein;Estrogen-regulated 24 kDa protein;Heat shock 27 kDa protein;HSP 27;Stress-responsive protein 27;SRP27 |
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Product Overview
U-Blot® HSP27 (Phospho Ser82) Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against HSP27. Recognizes Phospho (Ser82). It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | HSP27 |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT1395R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Protein A |
| Specificity | HSP27 (Phospho Ser82) Antibody detects endogenous levels of HSP27 protein only when phosphorylated at S82.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):QLsSG |
| Molecular Weight | Calculated: 23 kDa | Observed: 27 kDa |
| UniProt / Swiss-Prot | P04792 (Human) | P14602 (Mouse) | P42930 (Rat) |
Recommended dilutions: IHC 1:100-1:500;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Immunogen / Antigen
Heat shock protein beta-1
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:100-1:500;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT1395R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
