U-Blot® HSP40 Rabbit Polyclonal Antibody

SKU:BHA21807377
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-HSP40 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human, Mouse, Rat.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Options selector
Catalog no. Size
WP12762-50 50 uL
WP12762-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target HSP40
Antigen
  • DnaJ homolog subfamily B member 1
Alternative names DNAJB1;DNAJ1;HDJ1;HSPF1;DnaJ homolog subfamily B member 1;DnaJ protein homolog 1;Heat shock 40 kDa protein 1;HSP40;Heat shock protein 40;Human DnaJ protein 1;hDj-1
Gene ID 3337 (Human) | 81489 (Mouse)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • ELISA
Immunogen The antiserum was produced against synthesized peptide derived from human HSP40. AA range:271-320
Conjugate
  • Unconjugated
Molecular weight Observed: 45 kDa
Purification Affinity Purified
Cellular localization Cytoplasm . Nucleus . Nucleus, nucleolus . Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP12762
Main SKU BHA21807377
Antibody

Product Overview

U-Blot® HSP40 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against HSP40. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.

Validated Applications

Target HSP40
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purification Affinity Purified
Specificity HSP40 Polyclonal Antibody detects endogenous levels of HSP40 protein.
Molecular Weight Observed: 45 kDa
UniProt / Swiss-Prot P25685 (Human) | Q9QYJ3 (Mouse)

Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human HSP40. AA range:271-320

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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