| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | NFKBIA;IKBA;MAD3;NFKBI;NF-kappa-B inhibitor alpha;I-kappa-B-alpha;IkB-alpha;IkappaBalpha;Major histocompatibility complex enhancer-binding protein MAD3 |
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| Host | |
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| Isotype | |
| Reactivity | |
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Product Overview
U-Blot® IκB-α (Phospho Ser36) Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against IκB-α. Recognizes Phospho (Ser36). It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | IκB-α |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT1061R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Protein A |
| Specificity | IκB-α (Phospho Ser36) Monoclonal Antibody detects endogenous levels of IκB-α only when phosphorylated at Ser36.and dually phosphorylated at two sites.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):LDsMK |
| Molecular Weight | Calculated: 36 kDa | Observed: 39 kDa |
| UniProt / Swiss-Prot | P25963 (Human) | Q9Z1E3 (Mouse) | Q63746 (Rat) |
Recommended dilutions: IHC 1:500-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Immunogen / Antigen
NF-kappa-B inhibitor alpha
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:500-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT1061R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
