| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | IDE;Insulin-degrading enzyme;Abeta-degrading protease;Insulin protease;Insulinase;Insulysin |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® IDE Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against IDE. It has been validated for Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | IDE |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT1295R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunofluorescence, ELISA |
| Purification | Protein A |
| Specificity | Endogenous |
| Molecular Weight | Calculated: 118 kDa | Observed: 118 kDa |
| UniProt / Swiss-Prot | P14735 (Human) | Q9JHR7 (Mouse) | P35559 (Rat) |
Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Immunogen / Antigen
Insulin-degrading enzyme
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been describe
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT1295R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
