| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | IGF1R;Insulin-like growth factor 1 receptor;Insulin-like growth factor I receptor;IGF-I receptor;CD antigen CD221;INSR;Insulin receptor;IR;CD antigen CD220 |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® IGF1R (Phospho Tyr1135/1136)/Insulin Receptor β (Phospho Tyr1150/1151) Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against IGF IR. Recognizes Phospho (Tyr1135/1136/Tyr1150/1151). It has been validated for Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | IGF IR |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0867R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunofluorescence, ELISA |
| Purification | Protein A |
| Specificity | Antibody detects endogenous levels of IGF-I receptor and insulin receptor only when phosphorylated at tyrosine 1135/1136 or tyrosine 1150/1151, respectively. The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites): MAyLN |
| Molecular Weight | Calculated: 156 kDa | Observed: 95 kDa |
| UniProt / Swiss-Prot | P06213;P08069 (Human) | P24062;P15127 (Rat) |
Recommended dilutions: WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Immunogen / Antigen
Insulin-like growth factor 1 receptor
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA. Recommended dilutions: WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0867R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
