| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | IKZF1;IK1;IKAROS;LYF1;ZNFN1A1;DNA-binding protein Ikaros;Ikaros family zinc finger protein 1;Lymphoid transcription factor LyF-1 |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human Ikaros (C-terminus) protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Ikaros Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Ikaros. It has been validated for Western Blot and reacts with Human, Mouse, Dog.
Validated Applications
| Target | Ikaros |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Dog |
| Applications | Western Blot |
| Purification | Affinity Purified |
| Specificity | Ikaros Monoclonal Antibody detects endogenous levels of Ikaros protein. |
| Molecular Weight | Calculated: 58 kDa |
| UniProt / Swiss-Prot | Q13422 (Human) | Q03267 (Mouse) |
Recommended dilutions: WB 1:1000-1:2000;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant human Ikaros (C-terminus) protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Dog. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding
Safety & Handling
This antibody is validated for Western Blot. Recommended dilutions: WB 1:1000-1:2000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Dog. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
