| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | IL7R;Interleukin-7 receptor subunit alpha;IL-7 receptor subunit alpha;IL-7R subunit alpha;IL-7R-alpha;IL-7RA;CDw127;CD antigen CD127 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IL-7R/CD127 around the phosphorylation site of Tyr449. AA range:410-459 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® IL-7R (Phospho Tyr449) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against IL-7R. Recognizes Phospho. It has been validated for Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | IL-7R |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-IL-7R (Y449) Polyclonal Antibody detects endogenous levels of IL-7R protein only when phosphorylated at Y449. |
| Molecular Weight | Observed: 60 kDa |
| UniProt / Swiss-Prot | P16871 (Human) | P16872 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human IL-7R/CD127 around the phosphorylation site of Tyr449. AA range:410-459
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found. [provided by RefSeq, Dec 2015]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
