| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | IRF3;Interferon regulatory factor 3;IRF-3 |
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| Clonality | |
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| Reactivity | |
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Product Overview
U-Blot® IRF3 (Phospho Ser396) Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against IRF-3. Recognizes Phospho (Ser396). It has been validated for Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | IRF-3 |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0875R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, Immunofluorescence, ELISA |
| Purification | Protein A |
| Specificity | IRF3 (Phospho Ser396) Antibody detects endogenous levels of IRF3 protein only when phosphorylated at S396.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):HIsNS |
| Molecular Weight | Calculated: 47 kDa | Observed: 60 kDa |
| UniProt / Swiss-Prot | Q14653 (Human) | P70671 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Immunogen / Antigen
Interferon regulatory factor 3
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0875R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
