| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | MAPK8;JNK1;PRKM8;SAPK1;SAPK1C;Mitogen-activated protein kinase 8;MAP kinase 8;MAPK 8;JNK-46;Stress-activated protein kinase 1c;SAPK1c;Stress-activated protein kinase JNK1;c-Jun N-terminal kinase 1;MAPK9;JNK2;PRKM9;SAPK1A;Mi |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human JNK1/2/3 around the phosphorylation site of Thr183 and Tyr185. AA range:151-200 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® JNK1/2/3 (Phospho Thr183/Y185) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against JNK1/2/3. Recognizes Phospho. It has been validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA and reacts with Human, Mouse, Rat, Chicken, Pig, Fish.
Validated Applications
| Target | JNK1/2/3 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Chicken, Pig, Fish |
| Applications | Immunofluorescence, Western Blot, Immunohistochemistry, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-JNK1/2/3 (T183/Y185) Polyclonal Antibody detects endogenous levels of JNK1/2/3 protein only when phosphorylated at T183/Y185. |
| Molecular Weight | Observed: 46 kDa,54 kDa |
| UniProt / Swiss-Prot | P45983;P45984;P53779 (Human) | P49185;P49186;P49187 (Rat) |
Recommended dilutions: IF 1:50-200;WB 1:500-2000;IHC 1:50-300
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human JNK1/2/3 around the phosphorylation site of Thr183 and Tyr185. AA range:151-200
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Chicken, Pig, Fish. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spl
Safety & Handling
This antibody is validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA. Recommended dilutions: IF 1:50-200;WB 1:500-2000;IHC 1:50-300
Confirmed reactivity: Human, Mouse, Rat, Chicken, Pig, Fish. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
