U-Blot® Ki-67 Rabbit Polyclonal Antibody

SKU:BHA21807547
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-Ki-67 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human.
Clonality Polyclonal
Host Rabbit
Reactivity Human
Conjugate Unconjugated
Isotype IgG
Applications Immunohistochemistry, Immunofluorescence, ELISA
Options selector
Catalog no. Size
WP12917-50 50 uL
WP12917-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target Ki-67
Antigen
  • Ki 67
Alternative names MKI67;Antigen KI-67
Gene ID 4288 (Human)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
Applications
  • Immunohistochemistry
  • Immunofluorescence
  • ELISA
Immunogen The antiserum was produced against synthesized peptide derived from human Ki67. AA range:3207-3256
Conjugate
  • Unconjugated
Molecular weight Observed: 360 kDa
Purification Affinity Purified
Cellular localization Chromosome . Nucleus . Nucleus, nucleolus . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106). .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP12917
Main SKU BHA21807547
Antibody

Product Overview

U-Blot® Ki-67 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Ki-67. It has been validated for Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human.

Validated Applications

Target Ki-67
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human
Applications Immunohistochemistry, Immunofluorescence, ELISA
Purification Affinity Purified
Specificity Ki-67 Polyclonal Antibody detects endogenous levels of Ki-67 protein.
Molecular Weight Observed: 360 kDa
UniProt / Swiss-Prot P46013 (Human)

Recommended dilutions: IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human Ki67. AA range:3207-3256

Reactivity Notes

Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.

Scientific Background

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.

What species does it react with?

Confirmed reactivity: Human. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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