| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | XRCC6;G22P1;X-ray repair cross-complementing protein 6;5'-deoxyribose-5-phosphate lyase Ku70;5'-dRP lyase Ku70;70 kDa subunit of Ku antigen;ATP-dependent DNA helicase 2 subunit 1;ATP-dependent DNA helicase II 70 kDa subunit;CTC box-binding factor 75 kDa subunit;CTC75;CTCBF;DNA repair protein XRCC6;Lupus Ku autoantigen protein p70;Ku70;Thyroid-lupus autoantigen;TLAA;X-ray repair complementing defective repair in Chinese hamster cells 6 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized acetyl-peptide derived from the human Ku-70 around the acetylation site of K539. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Ku-70 (Acetyl Lys539) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Ku70/XRCC6. Recognizes Acetyl. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Rat, Mouse.
Validated Applications
| Target | Ku70/XRCC6 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Acetyl-Ku-70 (K539) Polyclonal Antibody detects endogenous levels of Ku-70 protein only when acetylated at K539. |
| Molecular Weight | Observed: 70 kDa |
| UniProt / Swiss-Prot | P12956 (Human) | P23475 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:20000;IF 1:50-200
Immunogen / Antigen
Synthesized acetyl-peptide derived from the human Ku-70 around the acetylation site of K539.
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:20000;IF 1:50-200
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
