| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Serine/threonine-protein kinase LATS1;Large tumor suppressor homolog 1;WARTS protein kinase;h-warts |
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| Host | |
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| Isotype | |
| Reactivity | |
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| Conjugate | |
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Product Overview
U-Blot® LATS1 (Phospho Thr1079) Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against LATS1. Recognizes Phospho (Thr1079). It has been validated for Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | LATS1 |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT1358R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, Immunofluorescence, ELISA |
| Purification | Protein A |
| Specificity | LATS1 (Phospho Thr1079) Antibody detects endogenous levels of LATS1 only when phosphorylated at Thr1079.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):EFtFR |
| Molecular Weight | Calculated: 127 kDa | Observed: 130 kDa |
| UniProt / Swiss-Prot | O95835 (Human) | Q8BYR2 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Immunogen / Antigen
LATS1 (Thr1079)
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatmen
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT1358R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
