| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Microtubule-associated proteins 1A/1B light chain 3A;Autophagy-related protein LC3 A;Autophagy-related ubiquitin-like modifier LC3 A;MAP1 light chain 3-like protein 1;MAP1A/MAP1B light chain 3 A;MAP1A/MAP1B LC3 A;Microtubule-associated protein 1 light chain 3 alpha |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthetic Peptide of LC3A |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® LC3A Mouse Monoclonal Antibody (5G10) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against LC3A. It has been validated for Western Blot, Immunofluorescence, Immunohistochemistry and reacts with Human, Rat, Mouse.
Validated Applications
| Target | LC3A |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5G10 |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, Immunofluorescence, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | LC3A protein detects endogenous levels of LC3A |
| Molecular Weight | Observed: 14,16 kDa |
| UniProt / Swiss-Prot | Q9H492 (Human) | Q91VR7 (Mouse) | Q6XVN8 (Rat) |
Recommended dilutions: WB 1:1000-2000;IHC 1:100-200;IF 1:200
Immunogen / Antigen
Synthetic Peptide of LC3A
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, Immunohistochemistry. Recommended dilutions: WB 1:1000-2000;IHC 1:100-200;IF 1:200
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone 5G10.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
