U-Blot® LC3B Mouse Monoclonal Antibody (9H5)

SKU:BHA21802745
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-LC3B antibody (Mouse host, monoclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence with reactivity to Human, Bovine.
Clonality Monoclonal
Host Mouse
Reactivity Human, Bovine
Conjugate Unconjugated
Clone 9H5
Applications Western Blot, Immunohistochemistry, Immunofluorescence
Options selector
Catalog no. Size
WM1191-50 50 uL
WM1191-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target LC3B
Antigen
  • MAP1LC3B
Alternative names MAP1LC3B
Gene ID 81631 (Human)
Host Mouse
Clone 9H5
Clonality
  • Monoclonal
Reactivity
  • Human
  • Bovine
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
Immunogen Recombinant Protein of LC3B of MAP1LC3B
Conjugate
  • Unconjugated
Molecular weight Observed: 14,16 kDa
Purification Affinity Purified
Cellular localization Cytoplasmic vesicle, autophagosome membrane ; Lipid-anchor . Endomembrane system ; Lipid-anchor . Mitochondrion membrane ; Lipid-anchor . Cytoplasm, cytoskeleton . Cytoplasmic vesicle . LC3-II binds to the autophagic membranes. LC3-II localizes with the mitochondrial inner membrane during Parkin-mediated mitophagy (PubMed:28017329). Localizes also to discrete punctae along the ciliary axoneme. .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WM1191
Main SKU BHA21802745
Antibody

Product Overview

U-Blot® LC3B Mouse Monoclonal Antibody (9H5) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against LC3B. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence and reacts with Human, Bovine.

Validated Applications

Target LC3B
Host Mouse
Clonality Monoclonal
Clone 9H5
Conjugate Unconjugated
Reactivity Human, Bovine
Applications Western Blot, Immunohistochemistry, Immunofluorescence
Purification Affinity Purified
Specificity LC3B protein detects endogenous levels of MAP1LC3B
Molecular Weight Observed: 14,16 kDa
UniProt / Swiss-Prot Q9GZQ8 (Human) | Q9CQV6 (Mouse)

Recommended dilutions: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000;IF 1:50-200

Immunogen / Antigen

Recombinant Protein of LC3B of MAP1LC3B

Reactivity Notes

Confirmed cross-reactivity: Human, Bovine. Confirm suitability for untested species before use.

Scientific Background

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence. Recommended dilutions: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000;IF 1:50-200

What species does it react with?

Confirmed reactivity: Human, Bovine. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

What is the clone number?

Clone 9H5.

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