| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | HCLS1;HS1;Hematopoietic lineage cell-specific protein;Hematopoietic cell-specific LYN substrate 1;LckBP1;p75 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HS1 around the phosphorylation site of Tyr397. AA range:366-415 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Lck BP-1 (Phospho Tyr397) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Lck BP-1. Recognizes Phospho. It has been validated for Western Blot, Immunohistochemistry and reacts with Human, Rat, Mouse.
Validated Applications
| Target | Lck BP-1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | Phospho-Lck BP-1 (Y397) Polyclonal Antibody detects endogenous levels of Lck BP-1 protein only when phosphorylated at Y397. |
| Molecular Weight | Observed: 55 kDa |
| UniProt / Swiss-Prot | P14317 (Human) | P49710 (Mouse) |
Recommended dilutions: WB 1:500-2000;IHC 1:50-300
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human HS1 around the phosphorylation site of Tyr397. AA range:366-415
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
developmental stage:Expressed in early stage of myeloid and erythroid differentiation.,function:Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.,PTM:Phosphorylated by LYN; rapidly after cross-linking of surface IgM on B-cells.,similarity:Contains 1 SH3 domain.,similarity:Contains 4 cortactin repeats.,subunit:Associates with the SH2 and SH3 domains of LCK. Binding to he LCK SH3 domain occurs constitutively, while binding to the LCK SH2 domain occurs only upon TCR stimulation. A similar binding pattern was observed with LYN, but not with FYN in which the FYN SH2 region associates upon TCR stimulation but the FYN SH3 region does not associate regardless of TCR stimulation. Directly associates with HAX1, through binding to its C-terminal region. Interacts with HS1BP3.,tissue specificity:Expressed only in tissues and cells of hematopoietic origin.
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry. Recommended dilutions: WB 1:500-2000;IHC 1:50-300
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
