U-Blot® Lfc Rabbit Polyclonal Antibody (WP12993)

SKU:BHA21807631
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-Lfc antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human, Rat, Mouse.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Rat, Mouse
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Options selector
Catalog no. Size
WP12993-50 50 uL
WP12993-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target Lfc
Antigen
  • Rho guanine nucleotide exchange factor 2
Alternative names ARHGEF2;KIAA0651;LFP40;Rho guanine nucleotide exchange factor 2;Guanine nucleotide exchange factor H1;GEF-H1;Microtubule-regulated Rho-GEF;Proliferating cell nucleolar antigen p40
Gene ID 9181 (Human)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Rat
  • Mouse
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • ELISA
Immunogen The antiserum was produced against synthesized peptide derived from in-ternal human ARHGEF2. AA range:383-432
Conjugate
  • Unconjugated
Molecular weight Observed: 101 kDa
Purification Affinity Purified
Cellular localization Cytoplasm, cytoskeleton . Cytoplasm . Cell junction, tight junction . Golgi apparatus . Cytoplasm, cytoskeleton, spindle . Cell projection, ruffle membrane . Cytoplasmic vesicle . Localizes to the tips of cortical microtubules of the mitotic spindle during cell division, and is further released upon microtubule depolymerization (PubMed:15827085). Recruited into membrane ruffles induced by S.flexneri at tight junctions of polarized epithelial cells (PubMed:19043560). Colocalized with NOD2 and RIPK2 in vesicles and with the cytoskeleton (PubMed:21887730). .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP12993
Main SKU BHA21807631
Antibody

Product Overview

U-Blot® Lfc Rabbit Polyclonal Antibody (WP12993) is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Lfc. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Rat, Mouse.

Validated Applications

Target Lfc
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Rat, Mouse
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purification Affinity Purified
Specificity Lfc Polyclonal Antibody detects endogenous levels of Lfc protein.
Molecular Weight Observed: 101 kDa
UniProt / Swiss-Prot Q92974 (Human) | Q60875 (Mouse)

Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from in-ternal human ARHGEF2. AA range:383-432

Reactivity Notes

Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.

Scientific Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200

What species does it react with?

Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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